The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR goods ended up digested and cloned into a pET21a+ vector applying NdeI and XhoI restriction enzymes. A degree mutation was introduced by the fast improve https://kemalj655whr7.wikidank.com/user